ABG = arterial blood gas
- pH, PaO2 & PaCO2 are all directly measured
- HCO3-, base excess, SaO2 are derived
PaO2 = partial pressure (tension) of O2 in arterial blood
Oxygen Tension Methods
(1) oxygen (Clarke’s) electrode: amount of O2 producing a voltage
(2) transcutaneous electrodes
(3) fluorescence-based blood gas analysis: filtration of xenon light being proportional to O2 tension
(4) ion selective or pH electrode
O2 Content and Capacity Methods
(2) blood haemolysis
(3) galvanic cell
(4) calorimetric method
- CO2 diffuses through a selectively permeable membrane of silicone rubber or Teflon into an aqueous electrolyte (NaHCO3).
- this causes a change in the H+ ion concentration -> measured by pH sensitive glass electrode.
- output voltage is logarithmically related to PCO2 in blood.
- negative log of H+ activity
- measured by pH sensitive glass with oxides of silicone, lithium & calcium, porous only to H+.
- the glass has its metal cations displaced by the H+ ions in the test solution.
-> development of an EMF and can be calculated by a modification of the Nernst equation.
-> log [H+] in the sample of blood when connected to a reference electrode of mercury beads suspended in mercurous chloride (HgCl2) and surrounded by a saturated KCl solution.
-> this potential is compared with the potential developed using a standard solution of selected pH value.
- derived from the Henderson–Hasselbalch equation
Base excess (BE)
- = amount of titratable acid in mmol/L needed to titrate one litre of blood to a pH of 7.4.
- with Hb of 150g/L
- PCO2 of 40mmHg
- @ 37C
-> represents the non-respiratory component of a pH disturbance.
Standard Base Excess (SBE)
- same as above but corrected for haemoglobin as Hb is a buffer of acid (more accurately reflects the BE in the ECF)
- ratio of oxyHb to total Hb
- normal 95 to 98%
- a laboratory test involving a blood sample heated to 37 C and subjected to light of various length and assesses absorption spectra.
- does not require pulsatile flow
- measures MetHb, COHb and other forms of Hb
- uses many other wavelengths
- measures either venous, arterial or capillary oxygenation
- contamination of line with flush solution
- extreme leukocytosis -> pseudohypoxaemia (from excessive in vitro O2 consumption)
- ice storage in polypropylene syringes (rather than glass) -> artefactual PaCO2 elevation
- inter-analyser variability
- inadequate heparinization
- non-linearity of Clark electrode when PaO2 > 150mmHg
- lack of appropriate electrolyte temperature
- interference of NO and halothane
- poor quality control
- ABG tensions fluctuate constantly even in stable patients
References and Links
Chris is an Intensivist and ECMO specialist at the Alfred ICU in Melbourne. He is also the Innovation Lead for the Australian Centre for Health Innovation at Alfred Health, a Clinical Adjunct Associate Professor at Monash University, and the Chair of the Australian and New Zealand Intensive Care Society (ANZICS) Education Committee. He is a co-founder of the Australia and New Zealand Clinician Educator Network (ANZCEN) and is the Lead for the ANZCEN Clinician Educator Incubator programme. He is on the Board of Directors for the Intensive Care Foundation and is a First Part Examiner for the College of Intensive Care Medicine. He is an internationally recognised Clinician Educator with a passion for helping clinicians learn and for improving the clinical performance of individuals and collectives.
After finishing his medical degree at the University of Auckland, he continued post-graduate training in New Zealand as well as Australia’s Northern Territory, Perth and Melbourne. He has completed fellowship training in both intensive care medicine and emergency medicine, as well as post-graduate training in biochemistry, clinical toxicology, clinical epidemiology, and health professional education.
He is actively involved in in using translational simulation to improve patient care and the design of processes and systems at Alfred Health. He coordinates the Alfred ICU’s education and simulation programmes and runs the unit’s education website, INTENSIVE. He created the ‘Critically Ill Airway’ course and teaches on numerous courses around the world. He is one of the founders of the FOAM movement (Free Open-Access Medical education) and is co-creator of LITFL.com, the RAGE podcast, the Resuscitology course, and the SMACC conference.
His one great achievement is being the father of two amazing children.
On Twitter, he is @precordialthump.